C0371
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 | Immunofluorescence Nuclear and cytoplasmic staining of cullin-4A in HeLa cells. Fixed cells were stained with 10 μg/mL Rabbit Anti-Cullin-4A (Cat. No. C0371) followed by Goat Anti-Rabbit IgG, FITC conjugate (x40 magnification). |
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C0371
Sigma
Anti-Cullin-4A antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Cul4A |
Description
| Immunogen | synthetic peptide corresponding to amino acids 46-65 of human cullin-4A, conjugated to KLH. The corresponding sequence is identical in rat cullin-4A. |
| Physical form | solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~89 kDa |
| species reactivity | human |
| concentration | ~1.5 mg/mL |
| application(s) | immunoblotting: 1-2 μg/mL using HEK-293T cells expressing human Cullin-4A |
| indirect immunofluorescence: 10-20 μg/mL using HeLa cells | |
| Gene Information | human ... CUL4A(8451) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 1 |
